By Rudolf Pribil
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Additional resources for Applied Complexometry
The hypsochromic effect produced by formation of the calcium che late can be improved by suppressing the colour of free indicator by addition of 20 - 30°/o of ethanol. Under these conditions the colour change in the calcium titration is from violet to nearly colourless. 2. Thymolph thale xone This indicator, 3'3 "-bis([N ,N-di (carboxyme thyl)amino]me thyl} thymolphthalein (XVIII) was first prepared by Korbl and Pribil . In strongly alkaline medium it forms intensely blue complexes with calcium, barium and strontium.
A great number of direct titrations with EDTA have been described. Because the chelates are only slightly soluble in water, very slow titration at elevated temperature is recommended. The colour change can be considerably improved by addition of 25 - 5 0 % of organic solvent such Sec. l] Complexometric Indicators 27 as ethanol, isopropyl alcohol, dioxan, dimethylformamide etc. . X: PAN In practical application PAN is not much used. Because of the solubility of its 1:1 and 1:2 complexes it has been applied in combination with EDTA to spectrophotometric determination of palladium, cobalt and uranium · 2.
According to Vytras and Langmyhr  such compounds contain 30 - 50°/o of the semi-condensation product - Semi-Glycinethymol Blue (SGTB). These authors described a new synthesis of GTB directly from Thymol Blue. The progress of the condensation was checked by paper chrotnatography. The GTB and its monosodium salt were purified by cation-exchange chromatography, and after evaporation yielded the Sec. 1 ] Compiexometrie Indicators 41 f r e e a c i d H^In. These a u t h o r s d e s c r i b e d a l l the a b s o r p t i o n s p e c t r a and measured a l l the a c i d - b a s e d i s s o c i a t i o n c o n s t a n t s , s t a r t i n g from t r i p l y p r o t o n a t e d a c i d Hyln3+: pK1 = - 2 .
Applied Complexometry by Rudolf Pribil